We hope that this simple synthetic phospholipid will offer novel perspectives in the development of more efficient and selective ion transporters.Ĭell is the basic unit of structure and function for all organisms. In addition, LC had obvious toxicity to HeLa cells, and the IC 50 was 100.0 μM after coculture for 36 h.
All these results help us to speculate that LC transports ions via a channel mechanism with a tetrameric aggregate as the active structure. It suggests that the sandwich interaction may play a key role in the ion transport across lipid bilayers. Both of the vesicle assay and patch clamp tests indicate that LC has selectivity for Rb +, whose ionic radius is larger than the cavity of crown ether. The voltage clamp measurement exhibits regular square-like current signals with considerably long opening times, which indicates that LC achieves efficient ion transport through a channel mechanism and its single channel conductivity is 17 pS.
Fluorescent vesicle assay shows that LC has an excellent transport activity, and the EC 50 value for K + is 11.2 μM. Inspired by the natural phospholipid structures for cell membrane, a synthetic phospholipid LC with an ion recognition group benzo-18-crown-6 (B18C6) moiety was prepared which has been demonstrated to be able to transport ions across the lipid bilayers. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.Chenxi Wang, Huiting Yang, Yanxin Xiang, Shihao Pang, Chunyan Bao * and Linyong Zhu
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